A Comparative Study of Three Detection Techniques for Leifsonia xyli Subsp. xyli, the Causal Pathogen of Sugarcane Ratoon Stunting Disease
Table 3
Ct values of RT-qPCR using serial dilution of Lxx-pMD18-T plasmid as templates.
Sample
2013.5.
2013.6.2
Mean Ct
Ct
Mean Ct
Blank (ddH2O)
—
—
—
—
Negative plasmid
—
—
—
—
100Lxx-pMD18-T plasmid
8.3205; 8.4208; 8.2091
8.3168
8.5207; 7.8205; 7.947
8.096
10−1Lxx-pMD18-T plasmid
11.5595; 11.4471; 11.3005
11.4357
11.2019; 11.4752; 10.9927
11.223
10−2Lxx-pMD18-T plasmid
13.515; 13.4772; 13.5749
13.5224
13.62; 13.7713; 13.5248
13.639
10−3Lxx-pMD18-T plasmid
14.7635; 14.6707; 14.6183
14.6842
16.1848; 16.1488; 16.2099
16.121
10−4Lxx-pMD18-T plasmid
18.0854; 18.1271; 18.0325
18.0817
20.161; 20.0562; 19.7764
19.998
10−5Lxx-pMD18-T plasmid
23.0183; 23.3196; —
23.169
24.3614; 24.4379; 23.9269
24.242
10−6Lxx-pMD18-T plasmid
25.0384; 24.8664; 24.7652
24.8900
28.0877; 28.1149; 27.6601
27.955
10−7Lxx-pMD18-T plasmid
29.1153; 19.8728; 29.2228
29.0703
30.9799; 30.3203; —
30.6501
10−8Lxx-pMD18-T plasmid
33.412; 32.8918; —
33.1519
34.906; 32.892; 33.412
33.737
10−9Lxx-pMD18-T plasmid
—
—
—
—
10−10Lxx-pMD18-T plasmid
—
—
—
—
Results of experiments conducted on 04/25/2013. Results of experiments conducted on 06/26/2013. “Ct” means cycle threshold. “—” means absence detection of Lxx.