The Associations between Biochemical and Microbiological Variables and Taste Differ in Whole Saliva and in the Film Lining the Tongue

Table 1

Primers used for qPCR assays.

Primer	Oligonucleotide sequence (-)	Target	Reference
SPU Fwd	AAACTCAAAKGAATTGACGG	All bacteria	a
SPU Rev	CTCACRRCACGAGCTGAC	All bacteria	a
ACT Fwd	TACGGCCGCAAGGCTA	Actinobacteria	a
ACT Rev	TCRTCCCCACCTTCCTCCG	Actinobacteria	a
BACT Fwd	CRAACAGGATTAGATACCCT	Bacteroidetes	a
BACT Rev	GGTAAGGTTCCTCGCGTAT	Bacteroidetes	a
FIRM Fwd	TGAAACTYAAAGGAATTGACG	Firmicutes	a
FIRM Rev	ACCATGCACCACCTGTC	Firmicutes	a
γ-P Fwd	TCGTCAGCTCGTGTYGTGA	γ-Proteobacteria	a
γ-P Rev	CGTAAGGGCCATGATG	γ-Proteobacteria	a
β-P Fwd	ACTCCTACGGGAGGCAGCAG	β-Proteobacteria	b
β-P Rev	TCACTGCTACACGYG	β-Proteobacteria	b
Fuso Fwd	CGCAGAAGGTGAAAGTCCTGTAT	Fusobacterium spp	c
Fuso Rev	TGGTCCTCACTGATTCACACAGA	Fusobacterium spp	c
Veil Fwd	A(C/T)CAACCTGCCCTTCAGA	Veillonella spp	d
Veil Rev	CGTCCCGATTAACAGAGCTT	Veillonella spp	d
Strep Fwd	GTACAGTTGCTTCAGGACGTATC	Streptococcus spp	e
Strep Rev	ACGTTCGATTTCATCACGTTG	Streptococcus spp	e
Prev Fwd	CACCAAGGCGACGATCA	Prevotella spp	f
Prev Rev	GGATAACGCCYGGACCT	Prevotella spp	f

De Gregoris et al., 2011; Pécastaings et al., 2016; Suzuki et al., 2004; Rinttilä et al., 2004; Picard et al., 2004; Marathe et al., 2012.