Degalactotigonin, a Steroidal Glycoside from Solanum nigrum, Induces Apoptosis and Cell Cycle Arrest via Inhibiting the EGFR Signaling Pathways in Pancreatic Cancer Cells

Figure 3

Effect of 1 on EGF-induced cell cycle progression of PANC1 cells. (a) Serum-starved PANC1 cells were pretreated with the indicated concentrations of 1 (0-3 μM) for 30 min and then stimulated with EGF (100 ng/mL) for 24 h, stained with propidium iodide, and analyzed using a flow cytometry. Histogram showed the percentage of cells within the sub-G1, G0/G1, S, and G2/M phases of the cell cycle. Data was expressed as means of at least three independent experiments. (b) Serum-starved PANC1 were incubated with EGF (100 ng/mL) for the indicated time and whole cell lysates were probed with the indicated antibodies. α-Tubulin was used as a loading control. (c) Serum-starved PANC1 cells were incubated with EGF (100 ng/mL) in the presence of the indicated concentrations of 1 for 3 hours and whole cell lysates were probed with the indicated antibodies. -Tubulin was used as a loading control.

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