AEG-1 Contributes to Metastasis in Hypoxia-Related Ovarian Cancer by Modulating the HIF-1alpha/NF-kappaB/VEGF Pathway

Figure 4

AEG-1, VEGF, NF-κB, and HIF-1α expression levels in ovarian carcinoma SKOV3 cells cultured under hypoxic conditions at different time points (0, 2, 4, 8, 10, 12, and 24 h). (a) After culture under hypoxic conditions for 8 h, AEG-1, HIF-1α, NF-κB, and VEGF protein amounts peaked () as determined by Western blotting. (b) After culture under hypoxic conditions for 8 h, AEG-1, HIF-1α, and VEGF mRNA amounts peaked () as determined by real-time PCR. Data are from three independent experiments.

(a)

(b)