Neuronal Proteomic Analysis of the Ubiquitinated Substrates of the Disease-Linked E3 Ligases Parkin and Ube3a

Figure 4

Proteomic analysis of Drosophila neuronal ubiquitome. A cumulative number of identified ubiquitinated proteins (green) isolated from Drosophila neurons by means of the approach are shown. A cumulative number of proteins that appear in control birA pulldowns, and which are therefore classified as background, are shown in red. The first analyses (until 2013) were obtained from fly embryonic developing neurons (using elav-GAL4 driver). Subsequent analyses were performed with proteins isolated from the Drosophila photoreceptor cells (employing GMR-GAL4 driver).