Apelin-13 Is an Early Promoter of Cytoskeleton and Tight Junction in Diabetic Macular Edema via PI-3K/Akt and MAPK/Erk Signaling Pathways

Table 1

Antibodies used in the present study.

Antibody	Cat. number	Source	Manufacturer	Western dilution	Immunofluorescence dilution
					HRMECs	Retina
Anti-actin	#4970S	rabbit	CST	1 : 1000
Anti-FAK	#3285S	rabbit	CST	1 : 1000
Anti-p-FAK	#8556S	rabbit	CST	1 : 1000
Anti-Src	#2108S	rabbit	CST	1 : 1000		1 : 100
Anti-p-Src	#6943S	rabbit	CST	1 : 1000
Anti-PLCγ1	#5690S	rabbit	CST	1 : 1000
Anti-p-PLCγ1	#8713S	rabbit	CST	1 : 1000
Anti-p38	#8690S	rabbit	CST	1 : 1000
Anti-p-p38	#4511S	rabbit	CST	1 : 1000
Anti-p-Akt	#4060S	rabbit	CST	1 : 1000
Anti-Akt	#4691S	rabbit	CST	1 : 1000
Anti-VEGF	#9698S	rabbit	CST	1 : 1000
Anti-p-VEGF	#2748S	rabbit	CST	1 : 1000
Anti-VE-Cadherin	ab33168	rabbit	Abcam	1 : 1000		1 : 200
Anti-occludin	No. 331588	mouse	Invitrogen	1 : 1000	1 : 1000	1 : 1000
Anti-ZO-1	ab150266	mouse	Abcam	1 : 1000	1 : 1000	1 : 1000
Anti-GAPDH	ab9484	mouse	Abcam	1 : 1000
Anti-apelin-13	ab59469	rabbit	Abcam	1 : 1000	1 : 500
Anti-APJ	ab84296	Rabbit	Abcam	1 : 1000
HRP-conjugated goat anti-rabbit IgG	#7074S	—	CST	1 : 5000
FITC-conjugated donkey anti-rabbit-tetramethyl rhodamine isothiocyanate	ab6881		Abcam		1 : 1000	1 : 1000
TRITC-conjugated donkey anti-rabbit-tetramethyl rhodamine isothiocyanate	ab6799		Abcam		1 : 1000	1 : 1000

CST: cell signaling technology (Danvers, MA, USA); Abcam (Cambridge, MA, USA); Invitrogen (Carlsbad, CA, USA); FAK: focal adhesion kinase; Akt: protein kinase B; Erk: extracellular signal-regulated kinase;VE-Cadherin: vascular endothelial- (VE-) Cadherin; ZO-1: zonula occludens-1; PLCγ1: phospholipase Cγ1; ; .