Site-Directed Spin Labeling EPR for Studying Membrane Proteins

<div>Three-pulse ESEEM FT data of <sup>2</sup>H-labeled d<sub>10</sub> Leu4 LRL8 peptide in TFE (<i>α</i>-helix) and DPPC liposomes (3<sub>10</sub>-helix) for <svg height="8.98583pt" id="M37" style="vertical-align:-0.3499308pt" version="1.1" viewbox="-0.0498162 -8.6359 23.3959 8.98583" width="23.3959pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M244 607C244 633 228 655 200 655C166 655 146 618 146 594C146 564 166 546 191 546C221 546 244 574 244 607ZM222 91L209 114C184 94 148 66 133 66C127 66 124 73 130 96L201 370C213 416 211 448 191 448C162 448 88 407 29 352L42 328C73 354 104 371 114 371C120 371 119 365 115 345L53 92C32 5 45 -12 68 -12C103 -12 186 50 222 91Z" id="g113-106"></path></g><g transform="matrix(.013,0,0,-0.013,6.455,0)"><path d="M535 230V280H323V490H265V280H52V230H265V-3H323V230H535Z" id="g117-36"></path></g><g transform="matrix(.013,0,0,-0.013,16.991,0)"><path d="M285 378C315 398 338 416 353 432C373 451 384 474 384 503C384 579 325 635 236 635H235C182 635 136 610 108 579L65 516L85 496C110 533 150 575 205 575C258 575 300 543 300 481C300 407 232 369 141 339L147 310C163 315 188 321 211 321C268 321 338 284 338 192C338 94 288 40 217 40C160 40 119 68 93 91C85 98 77 97 69 91C60 84 47 71 46 58C44 46 48 35 62 22C75 10 116 -12 162 -12C234 -12 424 62 424 224C424 297 373 359 285 376V378Z" id="g113-52"></path></g></svg> and <svg height="8.98583pt" id="M38" style="vertical-align:-0.3499308pt" version="1.1" viewbox="-0.0498162 -8.6359 23.3959 8.98583" width="23.3959pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M244 607C244 633 228 655 200 655C166 655 146 618 146 594C146 564 166 546 191 546C221 546 244 574 244 607ZM222 91L209 114C184 94 148 66 133 66C127 66 124 73 130 96L201 370C213 416 211 448 191 448C162 448 88 407 29 352L42 328C73 354 104 371 114 371C120 371 119 365 115 345L53 92C32 5 45 -12 68 -12C103 -12 186 50 222 91Z" id="g113-106"></path></g><g transform="matrix(.013,0,0,-0.013,6.455,0)"><path d="M535 230V280H323V490H265V280H52V230H265V-3H323V230H535Z" id="g117-36"></path></g><g transform="matrix(.013,0,0,-0.013,16.991,0)"><path d="M456 178V225H360V632H320C217 496 115 347 20 206V178H280V106C280 40 276 34 189 27V0H445V27C364 34 360 39 360 106V178H456ZM280 225H82C149 335 214 431 278 520H280V225Z" id="g113-53"></path></g></svg> samples (adapted from [<a href="/journals/bmri/2018/3248289/#B94">52</a>] with permission).</div>

BioMed Research International

Site-Directed Spin Labeling EPR for Studying Membrane Proteins

Figure 5