Molecular Cloning and Characterization of a Novel α-Amylase from Antarctic Sea Ice Bacterium Pseudoalteromonas sp. M175 and Its Primary Application in Detergent

Figure 2

The seven conserved regions of Amy175 with other known amylolytic enzymes from oligo-1,6-glucosidase (GH13 subfamilies 4, 16, 17, 18, 23, 29, 30, and 31), neopullulanase (GH13 subfamily 20), and GH13_36 subfamily. The characteristic sequence is highlighted as follows: entire family GH13-specific-blue; catalytic triad-yellow; oligo-1,6-glucosidase-specific-green; neopullulanase-specific-purple; intermediary GH13_36-specific-red. The name of an enzyme is composed of the GH13 subfamily number, source (organism), and the UniProt accession number.