Molecular Cloning and Characterization of a Novel α-Amylase from Antarctic Sea Ice Bacterium Pseudoalteromonas sp. M175 and Its Primary Application in Detergent

Figure 6

The effects of NaCl on the activity and stability of Amy175. (a) The effect of NaCl on the activity of Amy175 was measured at 25°C in 50 mM Tris-HCl buffer (pH 8.0) containing 0-5 M NaCl. The activity without NaCl was defined as 100%. (b) The effect of NaCl on stability was studied by preincubating enzymes at 25°C in 50 mM Tris-HCl buffer (pH 8.0) containing 0 or 1 M NaCl for the given times. The activity of Amy175 at 0 min was defined as 100%. Error bars represent the standard deviation of three independent measurements.

(a)

(b)