Molecular Cloning and Characterization of a Novel α-Amylase from Antarctic Sea Ice Bacterium Pseudoalteromonas sp. M175 and Its Primary Application in Detergent

Table 4

Comparison of Amy175 with other well-characterized cold-active α-amylases.

Source and α-amylase	Molecular weight (kDa)/size	Identity with Amy175	Temperature optimum (°C)	Residual activity at 0°C	pH optimum	NaCl (M) for optimum activity	Activators	Inhibitors	Reference
Pseudoalteromonas sp. M175 (Amy175)	62/550 aa	-	25	53.2%	8.0	1	DMSO, Mg^2+, Ni^2+, K^+ and; 1 mM Ca^2+, Ba^2+, Pb^2+	Cu^2+, Hg^2+, Mn^2+, Tween 80, β-ME	This study
Pseudoalteromonas haloplanktis TAB23; (AHA)	49/453 aa	26%	25	20%	7.0	0.5	-	-	[21]
Pseudoalteromonas arctica GS230; (ParAmy)	55/477 aa	26%	30	34.5%	7.5	-	Mn^2+, K^+, Na^+	Hg^2+, Cu^2+, Fe^3+	[22]
Pseudoalteromonas sp. MY-1 (rAmyA)	73/669 aa	-	40	-	7.0	-	K^+, Na^+, Ca^2+	-	[26]
Zunongwangia Profunda (AmyZ)	66/594 aa	26%	35	39%	7.0	1.5	Sr^2+, Fe^3+, Mg^2+, Ba^2+, , K^+	Cu^2+, Zn^2+, Mn^2+, Fe^3+, SDS, EDTA	[23]
Exiguobacterium sp. SH3 (AmyE)	53/509 aa	24%	30	4%	6.5	2	Triton X-100, Tween 20	Acetone, DMSO, Butanol	[25]
uncultured organism ()	56/486 aa	22%	10-15	70% at 1°C	8.0-9.0	-	Ca^2+	Cu^2+, Zn^2+, Ba^2+, Mg^2+, SDS, EDTA, Tween 20, Triton X-100	[27]
Geomyces pannorum	54/497 aa	-	40	over 20%	5.0	-	-	-	[40]
Arthrobacter agilis PAMC 27388	80/720 aa	-	30	-	3.0	-	Fe^3+, β-ME	, ammonium persulphate (APS), SDS, Triton X-100, urea	[38]