BioMed Research International

Research Article

miR-142-5p in Bone Marrow-Derived Mesenchymal Stem Cells Promotes Osteoporosis Involving Targeting Adhesion Molecule VCAM-1 and Inhibiting Cell Migration

Table 3

The sequences of reverse transcriptional primers and qPCR primers used in miRNA quantitative PCR.
PrimersSequences (5′-3′)
rno-miR-129-5p reverse transcription sequenceGTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGCAAGC
rno-miR-129-5p qPCR upstream primerGCGGCTTTTTGCGGTCTGG
rno-miR-146a-5p reverse transcription sequenceGTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAACCCA
rno-miR-146a-5p qPCR upstream primerGCGGTGAGAACTGAATTCCA
rno-miR-363-3p reverse transcription sequenceGTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACAGAT
rno-miR-363-3p qPCR upstream primerGCGGAATTGCACGGTATCC
rno-miR-206-3p reverse transcription sequenceGTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCCACAC
rno-miR-206-3p qPCR upstream primerGCGGTGGAATGTAAGGAAGT
rno-miR-142-3p reverse transcription sequenceGTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTCCATA
rno-miR-142-3p qPCR upstream primerGCGGTGTAGTGTTCCTACTT
rno-miR-142-5p reverse transcription sequenceGTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAGTAGT
rno-miR-142-5p qPCR upstream primerGCGGCATAAAGTAGAAAGC
rno-miR-223-5p reverse transcription sequenceGTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCAACTC
rno-miR-223-5p qPCR upstream primerGCGGCGTGTATTTGACAAGCT
rno-miR-223-3p reverse transcription sequenceGTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGGGGTA
rno-miR-223-3p qPCR upstream primerGCGGTGTCAGTTTGTCAAA
rno-miR-139-5p reverse transcription sequenceGTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCTGGAG
rno-miR-139-5p qPCR upstream primeGCGGTCTACAGTGCACGTGT
qPCR common downstream sequenceGTGCAGGGTCCGAGGT