Changes in Liver Gene Expression and Plasma Concentration of Rbp4, Fetuin-A, and Fgf21 in Sprague-Dawley Rats Subjected to Different Dietary Interventions and Bariatric Surgery

Table 1

Plasma levels of RBP4, fetuin-A, FGF21, and fold change of Rbp4, FetuinA (Ahsg), and Fgf21 in liver tissue 8 weeks after DJOS ( column) and SHAM ( column) surgery, subjected to 16 weeks of different dietary patterns, and intergroup comparison between DJOS and SHAM study groups ( column) using descriptive statistics and results of two-way analysis of variance.

	DJOS				SHAM				p ANOVA
Parameter	HF/HF	HF/CD	CD/HF	CD/CD	HF/HF	HF/CD	CD/HF	CD/CD	Group	Op.	Int.
Plasma levels
RBP-4 [ng/mL]	61.1 ± 10.4	40.5 ± 10.0	44.8 ± 10.2	11.5 ± 3.2	79.6 ± 11.7	54.4 ± 12.2	49.6 ± 21.1	17.7 ± 4.2	<0.001	<0.01	0.377
Fetuin-A [ng/mL]	12.6 ± 2.3	10.6 ± 4.9	11.4 ± 1.3	5.8 ± 2.2	16.2 ± 1.1	15.9 ± 1.5	14.0 ± 1.0	10.3 ± 1.7	<0.001	<0.001	0.498
FGF 21 [ng/mL]	304.8 ± 195.3	332.2 ± 285.5	442.3 ± 254.8	269.3 ± 139.5	772.9 ± 98.3	772.7 ± 74.1	721.1 ± 136.4	387.5 ± 130.8	<0.01	<0.001	<0.05
Fold change
RBP-4	1.26 ± 0.12	1.00 ± 0.08	1.17 ± 0.14	0.50 ± 0.09	1.57 ± 0.17	1.24 ± 0.23	1.74 ± 0.43	0.65 ± 0.22	<0.001	<0.001	<0.05
Fetuin-A (Ahsg)	1.60 ± 0.22	1.20 ± 0.21	0.58 ± 0.18	0.33 ± 0.10	2.94 ± 0.60	1.96 ± 0.34	1.46 ± 0.09	0.59 ± 0.19	<0.001	<0.001	<0.001
FGF 21	4.37 ± 0.55	4.44 ± 0.29	4.57 ± 0.40	1.07 ± 0.35	5.80 ± 0.40	5.31 ± 0.93	4.69 ± 1.14	1.11 ± 0.40	<0.001	<0.001	<0.01

Statistical significance was set at a p < 0.05. Abbreviations: RBP4: retinol binding protein 4; FGF21: fibroblast growth factor 21. DJOS: duodenal-jejunal omega switch surgery; HF: high-fat diet; CD: control diet; HF/HF, CD/HF, HF/CD, and CD/CD: type of diet 8 weeks before/8 weeks after surgery; Op.: operation type; Int.: interaction between group and operation type. Mean ± standard deviation or median (lower – upper quartile).