Changes in Liver Gene Expression and Plasma Concentration of Rbp4, Fetuin-A, and Fgf21 in Sprague-Dawley Rats Subjected to Different Dietary Interventions and Bariatric Surgery

Table 2

Multiple comparisons in contrast analysis of RBP4, fetuin-A (Ahsg), FGF21 plasma levels, and relative gene expression in liver tissue. Column 1: intergroup comparisons between HF/HF, CD/HF, HF/CD, and CD/CD groups of DJOS and SHAM. Column 2: intragroup comparisons between HF/HF, CD/HF, HF/CD, and CD/CD groups after DJOS surgery. Column 3: intragroup comparisons between HF/HF, CD/HF, HF/CD, and CD/CD groups after SHAM surgery.

Post-hoc

DJOS vs SHAM

DJOS

SHAM

1: HF/HF

2: HF/CD

3: CD/HF

4: CD/CD

1 vs2

1 vs 3

1 vs 4

2 vs 3

2 vs 4

3 vs 4

1 vs 2

1 vs 3

1 vs 4

2 vs 3

2 vs 4

3 vs 4

Plasma levels

RBP-4 [ng/mL]

<0.01

<0.01

0.473

0.318

<0.01

<0.05

<0.001

0.517

<0.001

<0.001

<0.001

<0.001

<0.001

0.471

<0.001

<0.001

Fetuin-A [ng/mL]

<0.01

<0.001

0.057

<0.001

0.121

0.349

<0.001

0.545

<0.001

<0.001

0.786

0.080

<0.001

0.150

<0.001

<0.01

FGF 21 [ng/mL]

<0.001

<0.001

<0.01

0.215

0.780

0.167

0.708

0.284

0.523

0.084

0.998

0.598

<0.001

0.613

<0.001

<0.01

Fold Change

RBP-4

<0.01

0.023

<0.001

0.149

<0.05

0.402

<0.001

0.096

<0.001

<0.001

<0.01

0.118

<0.001

<0.001

<0.001

<0.001

Fetuin-A (Ahsg)

<0.001

<0.001

<0.001

<0.05

<0.05

<0.01

<0.001

<0.001

<0.001

0.062

<0.001

<0.001

<0.001

<0.001

<0.001

<0.001

FGF 21

<0.001

<0.01

0.675

0.886

0.820

0.502

<0.001

0.656

<0.001

<0.001

0.103

<0.001

<0.001

<0.05

<0.001

<0.001

Post hoc analysis: statistical significance was set at a p < 0.05. Abbreviations: RBP4: retinol binding protein 4; FGF21: fibroblast growth factor 21; DJOS: duodenal-jejunal omega switch surgery; HF: high-fat diet; CD: control diet; HF/HF, CD/HF, HF/CD, and CD/CD: type of diet 8 weeks before/8 weeks after surgery.