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BioMed Research International
Volume 2018, Article ID 3501827, 7 pages
Research Article

In Vitro Lethal Activity of the Nematophagous Fungus Clonostachys rosea (Ascomycota: Hypocreales) against Nematodes of Five Different Taxa

1Área de Helmintología, CENID-Parasitología Veterinaria, INIFAP, Carretera Federal Cuernavaca-Cuautla No. 8534, Col. Progreso, 62500 Jiutepec, MOR, Mexico
2Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Carretera Amecameca-Ayapango, Km 2.5, 56700 Toluca de Lerdo, MEX, Mexico
3Unidad de Biotecnología, Centro de Investigación Científica de Yucatán (CICY-CONACYT), Chuburná de Hidalgo, No. 130, 972005 Mérida, YUC, Mexico
4INECOL, A.C. Red de Estudios Moleculares Avanzados, Unidad de Microscopía Avanzada del Clústec Científico y Tecnológico Biomimic, Carretera Antigua a Coatepec No. 351, Col. El Haya, 91070 Xalapa, VER, Mexico
5Universidad Autónoma de Campeche, Calle Avenida Agustín Melgar s/n, Buenavista, 24039 Campeche, CAM, Mexico

Correspondence should be addressed to Pedro Mendoza-de-Gives; moc.oohay@sevigdmordep and Liliana Aguilar-Marcelino; xm.bog.pafini@anailil.raliuga

Received 12 October 2017; Revised 18 January 2018; Accepted 4 February 2018; Published 20 March 2018

Academic Editor: Yann Quilichini

Copyright © 2018 Rosalia Rodríguez-Martínez et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This study was aimed to evaluate the in vitro lethal activity of the nematophagous fungi Clonostachys rosea against 5 nematodes species belonging to different taxa. Two groups of 35 Petri dishes (PD) each were divided into 5 series of 7 (PD). Group 1 (series 1, 2, 3, 4, and 5) contained only water agar; meanwhile group 2 plates (series 6, 7, 8, 9, and 10) contained C. rosea cultures growth on water agar. Every plate from the two groups was added with 500 nematodes corresponding to the following genera/specie: Haemonchus contortus, Caenorhabditis elegans, Rhabditis sp., Panagrellus redivivus, and Butlerius sp. After 5-day incubation at room temperature, free (nontrapped) larvae were recovered from plates using the Baermann funnel technique. Recovered nematodes were counted and compared with their proper controls. Results shown an important reduction percentage of the nematode population attributed to the fungal lethal activity as follows: H. contortus (L3) 87.7%; C. elegans 94.7%; Rhabditis sp. 71.9%; P. redivivus 92.7%; and Butlerius sp. 100% (). The activity showed by C. rosea against the H. contortus can be crucial for further studies focused to the biological control of sheep haemonchosis, although the environmental impact against beneficial nematodes should be evaluated.