Research Article

Synergistic Effects of Controlled-Released BMP-2 and VEGF from nHAC/PLGAs Scaffold on Osteogenesis

Figure 6

(a) Expression of osteogenic related signaling pathway proteins of MC3T3-E1 cells in nHAC/PLGAs scaffold. (b) Expression of col1 protein of MC3T3-E1 cells treated with LY294002 and SB203580. (c) Expression of Dlx-5 and osterix protein of MC3T3-E1 cells treated with SB203580. (d) Expression of osterix protein in nucleus and cytoplasm of MC3T3-E1 cells in BMP-2/VEGF group (con: control group; V: VEGF group; B: BMP-2 group; B+V: BMP-2/VEGF group; nu: nucleus, cy: cytoplasm).