Synergistic Effects of Controlled-Released BMP-2 and VEGF from nHAC/PLGAs Scaffold on Osteogenesis
Table 3
Proliferative activity (OD value) and ALP activity (DEA enzyme activity, μmol∙min−1∙g−1), relative expression of col1, Dlx-5, and osterix mRNA, relative expression of RUNX2, Dlx-5, osterix, and col1 protein of MC3T3-E1 cells in nHAC/PLGAs scaffold.
Time (days)
Control group
VEGF group
BMP-2 group
BMP-2/VEGF group
Proliferative activity
3
0.8507 ± 0.0317
1.3987 ± 0.1304##
0.7385 ± 0.1160##
1.1096 ± 0.0632
ALP activity
7
4.2738 ± 0.8482
4.6884 ± 0.0979##
29.8862 ± 1.4436##
38.0940 ± 2.1503
14
4.3097 ± 0.8896
4.8018 ± 1.0833##
47.5750 ± 3.0548##
59.1847 ± 1.8423
21
4.0477 ± 1.1751
4.9057 ± 1.1357##
54.8533 ± 2.9016##
78.0085 ± 6.8198
Relative expression of col 1 mRNA
7
1.0927 ± 0.0758
1.1907 ± 0.0939##
1.3614 ± 0.2367##
1.6776 ± 0.2768
14
1.1592 ± 0.1075
1.1095 ± 0.4211##
1.5429 ± 0.1926##
1.9635 ± 0.3183
21
0.9377 ± 0.0719
0.8569 ± 0.0447##
2.5563 ± 0.3960##
6.6649 ± 0.5074
Relative expression of col 1 protein
7
0.0935 ± 0.0269
0.1288 ± 0.0201
0.1551 ± 0.0235
0.1908 ± 0.0840
14
0.0968 ± 0.0043
0.0963 ± 0.0277##
0.3205 ± 0.0238##
0.6859 ± 0.0372
21
0.1182 ± 0.0278
0.1310 ± 0.0254##
0.4019 ± 0.0099##
0.7932 ± 0.0635
Relative expression of RUNX2 protein
7
0.0163 ± 0.0017
0.0258 ± 0.0082##
0.0968 ± 0.0057
0.0762 ± 0.0158
14
0.0210 ± 0.0026
0.0294 ± 0.0073##
0.1357 ± 0.0246
0.1401 ± 0.0260
21
0.0175 ± 0.0046
0.0292 ± 0.0064##
0.1930 ± 0.0832
0.1500 ± 0.0386
Relative expression of Dlx-5 mRNA
7
0.9742 ± 0.0921
1.0566 ± 0.0504##
2.0851 ± 0.2293
2.1265 ± 0.4104
14
1.0388 ± 0.0515
0.9120 ± 0.3276##
2.4911 ± 0.2984##
3.6416 ± 0.7365
21
0.9527 ± 0.0644
0.8845 ± 0.0413##
3.3096 ± 0.3557#
4.5190 ± 1.3563
Relative expression of Dlx-5 protein
7
0.0216 ± 0.0114
0.0348 ± 0.0012##
0.1017 ± 0.0254
0.1161 ± 0.0230
14
0.0253 ± 0.0064
0.0218 ± 0.0036##
0.0865 ± 0.0239#
0.1506 ± 0.0289
21
0.0291 ± 0.0029
0.0447 ± 0.0109##
0.2042 ± 0.0234##
0.4216 ± 0.0362
Relative expression of osterix mRNA
7
0.8809 ± 0.1061
0.7559 ± 0.0399#
1.2075 ± 0.1732
1.3938 ± 0.4592
14
1.0333 ± 0.0924
0.8624 ± 0.2732##
1.7024 ± 0.2401#
2.1167 ± 0.4100
21
0.8365 ± 0.4278
0.6243 ± 0.0860##
2.3860 ± 0.2616##
6.9909 ± 1.6734
Relative expression of osterix protein
7
0.0171 ± 0.0024
0.0153 ± 0.0045##
0.0400 ± 0.0048#
0.0814 ± 0.0182
14
0.0166 ± 0.0042
0.0209 ± 0.0028##
0.0947 ± 0.0147##
0.5140 ± 0.0287
21
0.0260 ± 0.0057
0.0243 ± 0.0036##
0.2001 ± 0.0269##
0.4832 ± 0.0376
Values are mean ± sd.P < 0.05 andP < 0.01 compared with control group. #P < 0.05 and ##P < 0.01 compared with BMP-2/VEGF group.