Biochanin A Induces S Phase Arrest and Apoptosis in Lung Cancer Cells

Figure 2

Biochanin A inhibits the proliferation of A549 and 95D cells. (a) Cells were treated with varying concentrations of Biochanin A, and the cell proliferation and IC 50 were determined by MTT assay on days 1, 2, and 3. Each value represents the mean ± SD (n = 3). (b) Biochanin A suppressed colony formation of A549 and 95D cells. Cells were treated with Biochanin A (0, 5, 10, and 20μmol/L) and were allowed to form colonies in fresh medium for 14 days. These results were from 1 representative experiment of 3 independent trials. The photomicrographic difference (left panel) and influence of colonies (mean ± SD, n = 3) (right panel) in colony formation are shown. < 0.05; < 0.01; < 0.001.

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(b)