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BioMed Research International
Volume 2018, Article ID 3589135, 8 pages
Research Article

The Potential Virulence Factors of Providencia stuartii: Motility, Adherence, and Invasion

1Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russia
2Interdisciplinary Center for Analytical Microscopy, Kazan Federal University, Kazan, Russia
3Institute of Cytology RAS, Saint Petersburg, Russia

Correspondence should be addressed to Naziia Kurmasheva; moc.liamg@7052ayizan

Received 25 September 2017; Revised 30 December 2017; Accepted 21 January 2018; Published 21 February 2018

Academic Editor: Isabel Sá-Correia

Copyright © 2018 Naziia Kurmasheva et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Providencia stuartii is the most common Providencia species capable of causing human infections. Currently P. stuartii is involved in high incidence of urinary tract infections in catheterized patients. The ability of bacteria to swarm on semisolid (viscous) surfaces and adhere to and invade host cells determines the specificity of the disease pathogenesis and its therapy. In the present study we demonstrated morphological changes of P. stuartii NK cells during migration on the viscous medium and discussed adhesive and invasive properties utilizing the HeLa-M cell line as a host model. To visualize the interaction of P. stuartii NK bacterial cells with eukaryotic cells in vitro scanning electron and confocal microscopy were performed. We found that bacteria P. stuartii NK are able to adhere to and invade HeLa-M epithelial cells and these properties depend on the age of bacterial culture. Also, to invade the host cells the infectious dose of the bacteria is essential. The microphotographs indicate that after incubation of bacterial P. stuartii NK cells together with epithelial cells the bacterial cells both were adhered onto and invaded into the host cells.