Calcitriol Prevents Cardiovascular Repercussions in Puromycin Aminonucleoside-Induced Nephrotic Syndrome

Figure 5

(a) Top: left ventricle mRNA and protein expression of TNF- in control (Ct-V and Ct-D) and nephrotic (PAN-V and PAN-D) rats, after 21 days of calcitriol (D) or vehicle (V) treatments. mRNA and protein expression were normalized for GAPDH gene and -actin protein, respectively. Results are expressed as % of control. versus Ct-V rats. Bottom: representative immunoblots of TNF- and -actin (25 KDa and 43 KDa bands, respectively). (b) Top: left ventricle mRNA and protein expression of IL-1 in control (Ct-V and Ct-D) and nephrotic (PAN-V and PAN-D) rats, after 21 days of calcitriol (D) or vehicle (V) treatments. mRNA and protein expression were normalized for GAPDH gene and -actin protein, respectively. Results are expressed as % of control. Bottom: representative immunoblots of IL-1 and -actin (31 KDa and 43 KDa bands, respectively).

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