Combination of Mitochondrial and Plasma Membrane Citrate Transporter Inhibitors Inhibits De Novo Lipogenesis Pathway and Triggers Apoptosis in Hepatocellular Carcinoma Cells

Figure 5

Effect of citrate transporter inhibitors on apoptosis and ΔΨm in primary human hepatocyte cells. Cells were treated with CTPi, PMCTi, and the combination of 0.4 mM of each inhibitor at IC₅₀ concentrations of each for 24 h. (a) Apoptotic cells were detected by flow cytometry and are shown in representative bar graph analysis. (b) Percentage of the loss of ΔΨm in primary human hepatocytes is shown in a bar graph. The control was defined as a vehicle for which cells were treated with a medium containing 0.2% DMSO alone. The CCCP was used as a positive control of the loss of ΔΨm. Data from at least three dependent experiments are expressed as mean ± SEM, .

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