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BioMed Research International
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2018
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Article
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Tab 1
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Research Article
Effect of Kisspeptin on the Developmental Competence and Early Transcript Expression in Porcine Oocytes Parthenogenetically Activated with Different Methods
Table 1
Primer sequences and product size used for real-time quantitative PCR.
Gene
Forward 3′→5′
Reverse 3′→5′
(°C)
Product size (bp)
Information
MOS
GGGAGCAACTGAACTTGGAG
AGAATGTTCGCTGGCTTCAG
60
115
NM_001113219 (accession number)
CCNB1
CAACTGGTTGGTGTCACTGC
TTCCATCTGCCTGATTTGGT
60
126
NM_001170768 (accession number)
GAPDH
ACACTCACTCTTCTACCTTTG
CAAATTCATTGTCGTACCAG
60
90
DQ845173.1 (GenBank)