Effect of Kisspeptin on the Developmental Competence and Early Transcript Expression in Porcine Oocytes Parthenogenetically Activated with Different Methods

Table 2

Embryonic development after activation of oocytes with electric pulse, ionomycin, and ethanol with or without supplementation of kisspeptin during 6-DMAP culture.

	Electric		Ionomycin		Ethanol
	KP+	KP−	KP+	KP−	KP+	KP−
Oocyte	150	150	150	150	150	150
Cleavage%
Blastocyst%
Fold change of	2.5	1.61	2.5	2.8	1.1	1.18
Fold change of	0.4	0.65	2.5	2.25	0.3	0.98

replicates with average 25 oocytes per each replicate. The proportion of cleavage and blastocyst was calculated for each replicate and data expressed as mean ± SEM. mean of fold change in MOS and CCNB1 expression in oocytes at 0 h and 4 h after activation = expression level at 4 h divided by the expression at 0 h (values are shown in Figure 2). carrying different superscripts are considered significant when .