Development of Two Analytical Methods Based on Reverse Phase Chromatographic and SDS-PAGE Gel for Assessment of Deglycosylation Yield in -Glycan Mapping

Figure 3

Quantification of level of deglycosylation in bovine fetuin (a–c) and RNase B (d–f) on reduced SDS-PAGE gel and RP-LC. Letter S represents Mark12 standard (2.5–200 kDa); a and d represent negative controls (glycoprotein sample solubilized in water replacing all deglycosylation reagents of PNGase-F enzyme, denaturant, and denaturation buffer); b and c represent deglycosylated bovine fetuin at 50 and 100 μg input; and e and f represent deglycosylated RNase B at 50 and 100 μg input.