BioMed Research International / 2018 / Article / Fig 1

Research Article

The Role of IL-6RA in UHMWPE Promotes Proliferation in Fibro-Like Synovial Cells

Figure 1

Culture and identification of fibroblast-like synovial cells. After primary separation and culture, the 5th generation cells were identified by flow cytometry and immunofluorescence. Morphological observation under light microscope (a). Identification of surface markers by flow cytometry (b). CD68 combined with DAPI staining (c). Vimentin combined with DAPI staining (d). The cells were observed on a fluorescence microscope (Olympus Corporation, Japan) at a magnification of 400x. Cell nuclei were visualized by DAPI staining in blue. The expression of vimentin was visualized by FITC staining in green. n=6.

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