The Role of IL-6RA in UHMWPE Promotes Proliferation in Fibro-Like Synovial Cells

Figure 3

Effects of UHMWPE on apoptosis of FLS cells. FLS were incubated with 0-1.0 g/L UHMWPE for 0, 1, and 7 d or incubated with 1.0 g/L UHMWPE for 0, 1, and 7 d. Apoptotic and necrotic cell populations were analyzed by flow cytometry; quantitative analysis of apoptotic cells after UHMWPE treatment, n=5 (a and b). The expression of apoptosis-related proteins in FLS cells was detected by Western blot assay and the changes of Bcl-2, Bax, caspase-3, and cleaved-caspase-3 were statistically analyzed, n=5 (c). Data was obtained from three independent experiments; results were shown as means ± standard deviation. < 0.05, compared with dose of 0 g/L; ^#p < 0.05, compared with 0 d.

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