BioMed Research International / 2018 / Article / Fig 5

Research Article

Osteogenesis-Related Behavior of MC3T3-E1 Cells on Substrates with Tunable Stiffness

Figure 5

MC3T3-E1 cell response to substrates with tunable stiffness observed in YAP protein level. (a) Immunofluorescent images of YAP in MC3T3-E1 plated on different substrates (ranging from 1:0 to 1:3, elastic modulus ranging from 2.7 MPa to 0.6 MPa) (scale bar = 50 μm). (b) YAP protein expression detected by western blotting and quantitative analysis by densitometry. YAP results were normalized to ACTB. p < 0.05 vs. 1:0, #p < 0.05 vs. 5:1, p< 0.05 vs. 1:1. (c) Quantitative RT-PCR analysis of RAP2 in MC3T3-E1 cultured on different substrates (n=3). All results were normalized against GAPDH, and the comparative threshold cycle method (ΔΔCt) was used to determine the relative mRNA levels. Data are shown as fold change in relation to the gene expression on 1:0 substrate.

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