Lats1/2-Mediated Alteration of Hippo Signaling Pathway Regulates the Fate of Bone Marrow-Derived Mesenchymal Stem Cells

Figure 4

The effects of high and low expression of Lats1 and Lats2 on the osteogenic and adipogenic differentiation of BMSCs. Mineralized bone matrix was visualized with Alizarin Red S staining ((a), 200×), Alizarin Red S was extracted from cells with cetylpyridinium chloride, and optical density (OD) at a wavelength of 570 nm was then measured (b), and osteogenic-specific gene (Runx2, OSX) expression was evaluated by qRT-PCR (c). The accumulation of neutral lipid vacuoles was visualized by Oil red O staining ((d), 200×), Oil red O was extracted from cells with isopropanol and optical density (OD) at a wavelength of 520 nm was then measured (e), and adipogenic-specific gene (C/EBPα, PPAR-) expression was evaluated by qRT-PCR (f) (n = 3; P < 0.05 vs. MSC-GFP, P < 0.05 vs. MSC-shcontrol).

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