Nanosecond Electric Pulses Induce Early and Late Phases of DNA Damage and Cell Death in Cisplatin-Resistant Human Ovarian Cancer Cells

<div>Apoptosis detected with flow cytometry after 2 and 8 h (<i>a, b</i>): Q4 represented early apoptotic cells, and Q2 represented late apoptotic cells; a higher apoptotic percentage was detected at 8 h, and the value in COC1 cells was higher than that in COC1/DDP cells. Activity of caspase-3 (<i>c</i>): relative light unit (RLU) reflected the enzymatic level; a higher level was noted at 8 h. HMGB1 level (<i>d</i>): no increase was detected. Data were mean ± standard deviation for 3 independent experiments. (a) versus control, p&lt;0.05; (b) versus 2 h, p&lt;0.05; (c) versus COC1 at the same time point, p&lt;0.05.</div>

BioMed Research International

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Figure 2

Figure 2: Nanosecond Electric Pulses Induce Early and Late Phases of DNA Damage and Cell Death in Cisplatin-Resistant Human Ovarian Cancer Cells 