Nanosecond Electric Pulses Induce Early and Late Phases of DNA Damage and Cell Death in Cisplatin-Resistant Human Ovarian Cancer Cells

Figure 3

Mitochondrial membrane potential detected with the JC-1 assay. Images under fluorescence microscopy (a): green fluorescence was due to the monomer of JC-1 and the number of green cells was increased at 8 h, indicating collapse of the potential. The membrane potential qualified with the ratio of fluorescence intensity (b): the potential was decreased at 8 h. Activity of caspase-9 at 2 and 8 h (c): relative light unit (RLU) reflected the enzymatic level; the enzymatic activation was detected at 8 h. Data were mean ± standard deviation for 3 independent experiments. The scale bar was 50 μm; (a) versus control, p<0.05.

(a)

(b)

(c)