Occurrence and Multilocus Genotyping of Giardia duodenalis in Yunnan Black Goats in China

Table 1

Primers used in the study; annealing temperatures used in the PCRs.

Gene	Primer	Sequences (5′-3′)	Annealing temperature (°C)	Reference
bg	GF1	AAGCCCGACGACCTCACCCGCAGTGC	55	[1]
	GR1	GAGGCCGCCCTGGATCTTCGAGACGAC
	GF2	GAACGAACGAGATCGAGGTCCG	55
	GR2	CTCGACGAGCTTCGTGTT
gdh	Gdh1	TTCCGTRTYCAGTACAACTC	50	[1]
	Gdh2	ACCTCGTTCTGRGTGGCGCA
	Gdh3	ATGACYGAGCTYCAGAGGCACGT	65
	Gdh4	GTGGCGCARGGCATGATGCA
tpi	AL3543	AAATIATGCCTGCTCGTCG	50	[1, 3]
	AL3546	CAA ACCTTITCCGCAAACC
	ALEf	CCCCTTCTGCCGTACATTTAT	58
	ALEr	GGCTCGTAAGCAATAACGACTT