Table of Contents Author Guidelines Submit a Manuscript
BioMed Research International
Volume 2018, Article ID 4607945, 11 pages
Research Article

Biodegradable Alginate-Chitosan Hollow Nanospheres for Codelivery of Doxorubicin and Paclitaxel for the Effect of Human Lung Cancer A549 Cells

1Nursing College, Jinzhou Medical University, 40 Songpo Road, Linghe, Jinzhou, Liaoning 121000, China
2Pharmacy School, Jinzhou Medical University, 40 Songpo Road, Linghe, Jinzhou, Liaoning 121000, China
3Department of Medical Oncology, First Affiliated Hospital of Jinzhou Medical University, 40 Songpo Road, Linghe, Jinzhou, Liaoning 121001, China

Correspondence should be addressed to Yu Gao; moc.361@6111_uyoag

Received 7 October 2017; Revised 28 November 2017; Accepted 19 December 2017; Published 28 January 2018

Academic Editor: Vesa-Pekka Lehto

Copyright © 2018 Liu Tao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A biodegradable alginate coated chitosan hollow nanosphere (ACHN) was prepared by a hard template method and used for codelivery of doxorubicin (DOX) and paclitaxel (PTX) to investigate the effect on human lung cancer A549 cells. PTX was loaded into the nanometer hollow structure of ACHN through adsorption method. DOX was coated on surface of ACHN through electrostatic interaction. Drug release studies exhibited a sustained-release effect. According to X-ray diffraction patterns (XRD), differential scanning calorimetry (DSC), and Fourier transform infrared spectroscopy (FT-IR) analysis, DOX structure in the loading samples (DOX-PTX-ACHN) was of amorphous state while PTX was microcrystalline. Cytotoxicity experiments showed ACHN was nontoxic as carrier material and the combination of DOX and PTX in DOX-PTX-ACHN exhibited a good inhibiting effect on cell proliferation. Cell uptake experiments demonstrated that DOX-PTX-ACHN accumulated in the cytoplasm. Degradation experiments illustrated that ACHN was a biodegradable material. In summary, these results clearly indicate that ACHN can be utilized as a potential biomaterial to transport multiple drugs to be used in combination therapy.