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BioMed Research International
Volume 2018, Article ID 4651639, 9 pages
Research Article

A Rapid Detection Method for Morphological Characteristics of Biological Cells Based on Phase Imaging

1Faculty of Science, Jiangsu University, Zhenjiang 212013, China
2School of Mechanical Engineering, Jiangsu University, Zhenjiang 212013, China

Correspondence should be addressed to Ying Ji; nc.ude.sju@yj

Received 20 October 2017; Revised 5 February 2018; Accepted 4 March 2018; Published 11 April 2018

Academic Editor: Toshiyuki Sawaguchi

Copyright © 2018 Wen-Bo Tang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This paper presents a rapid label-free method for the identification of morphological characteristics of biological cells. Based on quantitative phase microscopy as well as the connotation of phase value, the gradient operator of phase and the associated analytic processing are employed to determine the edge of different parts of the samples. A heterogeneous biological cell model is established by simulation to show the mechanism of this method and a polystyrene bead is selected as a sample to confirm its validity by optical experiment. The result agrees well with the actual situation and this approach is proved to have good antinoise ability. Furthermore, a neutrophil is investigated by this method. Based on the optical experiment and the related analysis, the basic structure characteristics of the cell are obtained. It is indicated that the method presented in this paper could be applied to rapid identification and classification of living cells.