Altered DNA Methylation of Long Noncoding RNA uc.167 Inhibits Cell Differentiation in Heart Development

Figure 1

Locations and functions of uc.167. (a) uc.167 expression levels in P19 cells were significantly higher than those of the NC groups. and , Student’s -test. (b) Optical microscopy and fluorescence microscopy (×100) were used to observe lentivirus transfection efficiency via GFP expression of the uc.167 overexpression vector and control vector in P19 cells after 48 h transfection. (c) qRT-PCR analyses of uc.167 in cytoplasmic and nuclear fractions from P19 cells. uc.167 mainly located in the nucleus. (d) Cardiac markers were decreased in the uc.167 overexpressed group compared with control group. versus NC groups. Student’s -test.

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