The Ethyl Acetate Extract of Gynura formosana Kitam. Leaves Inhibited Cervical Cancer Cell Proliferation via Induction of Autophagy

Figure 5

EAEG induced HeLa cells undergoing autophagic cell death. (a) Cell viability of HeLa cells treated with 60 μg/ml EAEG for 24 h in presence or absence of 5 mM 3MA. (b) Western blot analysis of autophagy and proliferation proteins. GAPDH served as the loading control. The protein relative ratio was calculated using ImageJ. The data were expressed as mean ± SEM (). with respect to the control group and with respect to EAEG treatment group. EAEG: ethyl acetate extract of Gynura formosana Kitam. leaves.

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