A Novel Thermostable GH3 β-Glucosidase from Talaromyce leycettanus with Broad Substrate Specificity and Significant Soybean Isoflavone Glycosides-Hydrolyzing Capability

Table 3

Conversion of soybean isoflavone glycosides into free isoflavones by Bgl3B and the commercial glucosidase (G4511) from Sigma-Aldrich.

Enzyme	Isoflavone glycosides (mg/ml)			Free isoflavones (mg/ml)
	Daidzin	Genistin	Glycitin	Daidzein	Genistein	Glycitein
Control	45.2 ± 1.9	80.5 ± 4.7	7.3 ± 1.2	26.4 ± 2.8	15.3 ± 1.6	1.8 ± 1.1
Bgl3B	2.2 ± 0.8	19.3 ± 4.2	1.8 ± 3.2	42.2 ± 2.7	26.5 ± 1.8	3.7 ± 0.4
G4511	6.7 ± 0.3	28.8 ± 2.7	2.7 ± 0.8	36.8 ± 1.5	20.4 ± 0.7	3.0 ± 0.7

Results are the mean of three replicates. The reaction systems without enzyme addition were treated as blank controls.