BioMed Research International / 2018 / Article / Tab 3

Research Article

A Novel Thermostable GH3 β-Glucosidase from Talaromyce leycettanus with Broad Substrate Specificity and Significant Soybean Isoflavone Glycosides-Hydrolyzing Capability

Table 3

Conversion of soybean isoflavone glycosides into free isoflavones by Bgl3B and the commercial glucosidase (G4511) from Sigma-Aldrich.

EnzymeIsoflavone glycosides (mg/ml)Free isoflavones (mg/ml)
DaidzinGenistinGlycitinDaidzeinGenisteinGlycitein

Control45.2 ± 1.980.5 ± 4.77.3 ± 1.226.4 ± 2.815.3 ± 1.61.8 ± 1.1
Bgl3B2.2 ± 0.819.3 ± 4.21.8 ± 3.242.2 ± 2.726.5 ± 1.83.7 ± 0.4
G45116.7 ± 0.328.8 ± 2.72.7 ± 0.836.8 ± 1.520.4 ± 0.73.0 ± 0.7

Results are the mean of three replicates. The reaction systems without enzyme addition were treated as blank controls.

Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.