The Native Monomer of Bacillus Pumilus Ribonuclease Does Not Exist Extracellularly

Figure 1

Electrophoretic study of binase. (a) Polyacrylamide gel electrophoresis (PAGE) with 0.1% SDS at nanoconcentrations of the enzyme (M, molecular weight markers). (b) Zymogram of binase at nanoconcentrations. (c) PAGE of binase at microconcentration (10 µg) in the presence of strong denaturing agents: 1 - 1.5 % SDS; 2 - 2 % SDS.

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(b)

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