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BioMed Research International
Volume 2018, Article ID 4898153, 8 pages
Research Article

Increased Micronuclei Frequency in Oral and Lingual Epithelium of Treated Diabetes Mellitus Patients

1Faculty of Odontology, Autonomous University of Sinaloa, 80010 Culiacán, SIN, Mexico
2Faculty of Biological and Chemical Sciences, Autonomous University of Sinaloa, 80010 Culiacán, SIN, Mexico
3Faculty of Biology, Autonomous University of Sinaloa, 80010 Culiacán, SIN, Mexico
4Boston Children’s Hospital and Harvard Medical School, Harvard University, Boston, MA 02115, USA

Correspondence should be addressed to Rosalío Ramos-Payán; xm.ude.sau@proilasor

Received 14 September 2017; Revised 1 November 2017; Accepted 19 November 2017; Published 9 January 2018

Academic Editor: Konstantinos Kantartzis

Copyright © 2018 Jesús Emilo Quintero Ojeda et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Diabetes mellitus (DM) is a metabolic disease characterized by persistent high levels of glucose in plasma. Chronic hyperglycemia is thought to increase oxidative stress and the formation of free radicals that in turn damage cells. Thus, we decided to determine the frequency of nuclear abnormalities in epithelial cells from cheek and tongue mucosa of DM patients with type 1 (DM1, treated only with insulin) and type 2 (DM2, treated with metformin) using the buccal micronucleus cytome (BMCyt) assay. Micronuclei frequency in cheek epithelial cells was higher in both DM1 ( ± , ) and DM2 ( ± , ) patients, as compared to healthy controls (0.07  ±  0.06). Similarly, micronuclei frequency in tongue epithelium was increased in DM1 (0.81  ±  0.22, ) and DM2 (0.41  ±  0.21, ) groups, in comparison to controls (0.06  ±  0.05). Besides, we found a positive correlation between micronuclei frequency and the onset time of DM2 in both cheek (ρ = 0.69, ) and tongue epithelial cells (ρ = 0.71, ), but not with onset time of DM1 or age of the patients. Considering all this, we pose that BMCyt could serve as a fast and easily accessible test to assess genotoxic damage during dental visits of DM patients, helping to monitor their disease.