T-614 Promotes Osteoblastic Cell Differentiation by Increasing Dlx5 Expression and Regulating the Activation of p38 and NF-κB

Figure 4

p38 signaling pathway in osteoblast differentiation in MSCs. MSCs were cultured in α-MEM supplemented with 10% FBS and 50 ng/ml rhBMP-2 in the absence or presence of 10 μg/ml T-614. Then, the cells were harvested at 1.5, 2.5, 3, 4, and 6 h to detect the expressions of phospho-p38 by Western blot. The data are representative of three experiments with similar results.