T-614 Promotes Osteoblastic Cell Differentiation by Increasing Dlx5 Expression and Regulating the Activation of p38 and NF-κB

Figure 5

The effect of T-614 on the NF-κB signal pathway. MSCs were cultured in α-MEM supplemented with 10% FBS and 20 ng/ml TNF-α in the absence or presence of 10 μg/ml T-614. Then, the cells were harvested at 0.75, 1, 1.5, 2, and 3 h to detect the expressions of NF-κB and p-NF-κB by Western blot. The data are representative of three experiments with similar results.