Validation of Housekeeping Genes as Reference for Reverse-Transcription-qPCR Analysis in Busulfan-Injured Microvascular Endothelial Cells

Figure 1

Examination of primer specificity and size of RT-qPCR amplification productions. (a) Melting curve analysis of the 14 reference genes amplicons after the RT-qPCR reactions. Only one peak for each primer was shown, suggesting high specificity of primers. (b) Examination of primer specificity and amplicon size. The high specificity with only one band as expected of each RT-qPCR amplification production using 2% agarose gel electrophoresis was shown.

(a)

(b)