Proteomic Analysis of Liver from Human Lipoprotein(a) Transgenic Mice Shows an Oxidative Stress and Lipid Export Response

Figure 4

Representative 2D-PAGE Western blot images of antioxidant proteins in wildtype and Lp(a) mice. Western blots were used to validate the comparative proteomic analysis between the Lp(a) and wildtype mice for antioxidant proteins. Pooled liver protein extracts (n = 12) were separated by 2D PAGE in triplicate and transferred onto nitrocellulose membrane. Each membrane was probed with one of the following primary antibodies: Sod1 (a, b), Gpx1 (c, d), or Prdx6 (e, f). The proteins of interest were detected with an HRP-conjugated secondary antibody and imaged on a LAS-3000 luminescent analyzer. Arrows indicate the protein spots that correspond to those identified by 2D-PAGE analysis.

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