Proteomic Analysis of Liver from Human Lipoprotein(a) Transgenic Mice Shows an Oxidative Stress and Lipid Export Response

Table 1

Proteins showing significant (P<0.05) differential expression in the livers of Lp(a) versus wildtype mice on a normal chow diet.

2D Gel Spot Number	Identified Protein; (Swiss-Prot Accession Number)	Fold Change	P value	Primary Function(s)
Oxidative Stress
1	Protein DJ-1 (Park7, Q99LX0)	2.3	< 0.05	Oxidative stress response
2	Peroxiredoxin 6 (Prdx6, O08709)	2.2	< 0.05	Peroxide metabolism
3	Peroxiredoxin 6 (Prdx6, O08709)	3.4	< 0.0001	Peroxide metabolism
4	Peroxiredoxin 6 (Prdx6, O08709)	1.9	< 0.05	Peroxide metabolism
5	Glutathione peroxidase 1 (Gpx1, Q5RJH8)	2.9	< 0.01	Peroxide metabolism
6	Superoxide dismutase 1 (Sod1, P08228)	1.9	< 0.05	Superoxide metabolism
7	Superoxide dismutase 1 (Sod1, P08228)	3.2	< 0.05	Superoxide metabolism
Lipid Metabolism
8	3-ketoacyl-CoA thiolase (Acaa2, Q8JZR8)	2.8	< 0.05	Fatty acid oxidation
9	Enoyl-CoA hydratase (Echs1, Q8BH95)	-2.6	< 0.01	Fatty acid oxidation
10	Apolipoprotein A-IV (Apoa4, P06728)	2.7	< 0.01	Lipid transport
11	Apolipoprotein A-I (ApoA1, Q00623)	4.0	< 0.05	Lipid transport
12	Fatty acid-binding protein 4; (Fabp4, P04117)	2.1	< 0.05	Fatty acid transport
Homeostatic Regulation
13	Antithrombin III (Serpinc1, P32261)	1.9	< 0.05	Blood coagulation
14	14-3-3 protein gamma (Ywhag, P61982)	2.2	< 0.05	Signal transduction
15	Phosphatidylethanolamine binding protein 1 (Pebp1, P70296)	1.6	< 0.001	Signal transduction
16	Carbonic anhydrase 3 (Ca3, P16015)	-1.9	< 0.01	pH homeostasis
17	Lactoylglutathione lyase (Glo1, Q9CPU0)	1.8	< 0.001	Methylglyoxal detoxification
Energy Metabolism
18	Cytochrome b-cl complex subunit 1 (Uqcrc1, Q00896)	4.2	< 0.05	Electron transport
19	Cytochrome b5 (Cyb5a, P56395)	3.2	< 0.05	Electron transport
20	ATP synthase subunit d; (Atp5h, Q9DCX2)	1.7	< 0.05	ATP synthesis
Metabolic Process
21	Inorganic pyrophosphatase (Ppa1, Q9D819)	1.9	< 0.05	Phosphate metabolism
22	3-hydroxy anthranilate 3,4-dioxygenase (Haao, Q78JT3)	1.5	< 0.05	Quinolinic acid metabolism
23	Pyridoxine-5’phosphate oxidase (Pnpo, Q91XF0)	2.7	< 0.01	Pyridoxine biosynthesis
Macromolecular Process
24	Translationally controlled tumor protein (Tpt1, P63028)	3.0	< 0.05	Microtubule stabilisation
25	Protein disulfide isomerase A3 (Pdia3, P27773)	1.6	< 0.05	Glycoprotein synthesis
26	60 kDa heat shock protein (Hspd1, P63038)	1.5	< 0.05	Protein folding
Unknown Primary Function
27	Phenazine biosynthesis-like domain containing protein 2 (Pbld2, Q9CXN7)	1.9	< 0.01	Unknown

Fold change between Lp(a) versus wildtype mice. Positive number indicates an increased expression in the Lp(a) mice. Negative number indicates a decreased expression in the Lp(a) mice. Proteins are categorised by function as determined by gene ontology (GO) analysis.