γδT Cells Exacerbate Podocyte Injury via the CD28/B7-1-Phosphor-SRC Kinase Pathway

Figure 4

(a) Western blotting analysis indicated higher phosphor-SRC expression in nephrotic WT mice group than control group and nephrotic TCRδ^−/− mice group. (b) Podocytes apoptosis rate detection by Annexin V-PI apoptosis detection kit. Both of the early and late apoptosis rate were analyzed. Compared to control group, the early apoptosis rate of Groups C and D both significantly increased (). However, compared to other groups, only Group D had higher late apoptosis rate (). Group E, which was treated with CTLA4-Ig, could alleviate both the early and late apoptosis of Group D. ((c) and (d)) The phosphor-SRC and B7-1 of Podocytes was detected by cyto-immunofluorescence; and . Phosphor-SRC expression was higher in Group D than other groups ( for Group D, compared with , , , and for Groups A, B, C, and E, resp., all ). B7-1 expression was induced by LPS (, , , and for Groups A to D, resp., all , and for Group E, compared to Group A as well, ); however, there was no significant difference of B7-1 expression in Groups B-E. (e) Immunofluorescence staining for F-actin (green).

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