Cytokine Effect of TLR3, TLR4, and TLR7 Agonists Alone or Associated with Leishmania infantum Antigen on Blood from Dogs

Table 1

Clinical, serological, molecular, and parasite specific IFN-γ production from each individual dog tested (n=24).

	SIGNALMENT			LSA IFN- (pg/mL); (INTERPRETATION)		ELISA UNITS; (INTERPRETATION)		RT-PCR; (parasite/mL); (INTERPRETATION)
	AGE (MONTHS)	BREED	SEX
1	96.0	FOX HOUND mixed	M	0.0	(NP)	5.2	(-)	0.5	(+)
2	24.0	FOX HOUND mixed	F	993.6	(P)	4.4	(-)	0.0	(-)
3	60.0	ARIEGE POINTER	F	10.7	(NP)	4.1	(-)	0.0	(-)
4	24.0	FOX HOUND mixed	M	0.0	(NP)	2.9	(-)	0.0	(-)
5	60.0	FOX HOUND mixed	M	41.5	(P)	4.2	(-)	0.0	(-)
6	72.0	GRIFFON NIVERNAIS	M	95.5	(P)	7.6	(-)	0.0	(-)
7	12.0	GRIFFON NIVERNAIS	M	314.2	(P)	7.9	(-)	0.5	(+)
8	48.0	ARIEGE POINTER	M	0.0	(NP)	6.1	(-)	0.2	(+)
9	72.0	IBIZAN HOUNDS	M	920.4	(P)	3.0	(-)	0.0	(-)
10	12.0	BRUNO DE JURA	F	0.0	(NP)	3.7	(-)	0.0	(-)
11	108.0	ENGLISH POINTER mixed	F	0.0	(NP)	10.1	(-)	0.0	(-)
12	48.0	DACHSHUNDS	M	10.0	(NP)	4.0	(-)	0.0	(-)
13	36.0	DACHSHUNDS	M	285.1	(P)	5.1	(-)	0.0	(-)
14	36.0	ENGLISH SETTER	F	142.9	(P)	4.9	(-)	0.7	(+)
15	48.0	IBIZAN HOUNDS	M	1635.0	(P)	81.4	(+)	0.0	(-)
16	36.0	IBIZAN HOUNDS	M	4663.0	(P)	34.5	(-)	0.0	(-)
17	24.0	IBIZAN HOUNDS	M	3196.0	(P)	16.6	(-)	0.0	(-)
18	8.0	IBIZAN HOUNDS	F	619.5	(P)	78.9	(+)	0.0	(-)
19	8.0	IBIZAN HOUNDS	F	14.8	(NP)	19.3	(-)	0.0	(-)
20	8.0	IBIZAN HOUNDS	F	0.0	(NP)	37.5	(+)	0.0	(-)
21	24.0	IBIZAN HOUNDS	F	0.0	(NP)	10.8	(-)	0.0	(-)
22	24.0	IBIZAN HOUNDS	F	119.7	(P)	21.3	(-)	N/D
23	72.0	IBIZAN HOUNDS	F	574.5	(P)	10.6	(-)	N/D
24	48.0	IBIZAN HOUNDS	M	165.9	(P)	16.8	(-)	N/D

Dogs that presented stage I papular dermatitis.
LSA: Leishmania soluble antigen, IFN-γ: Interferon-gamma, pg: picograms, mL: milliliter, ELISA: enzyme linked immunosorbent assay, RT-PCR: real time polymerase chain reaction, M: male, F: female, NP: non-IFN-γ producer, P: IFN-γ producer, (-): negative, (+): positive, and N/D: no data.