Inflammatory Cellular Response to Mechanical Ventilation in Elastase-Induced Experimental Emphysema: Role of Preexisting Alveolar Macrophages Infiltration

Figure 2

Morphometric analysis. (a), (b), (c) Representative photomicrographs (50-fold magnification) of histological slides of murine lungs. Hematoxylin-eosin staining. Saline SV mice, 21 days after tracheal instillation of saline (a), Elastase SV mice, D14 (b), Elastase SV mice, D21 (c). (d) Mean chord length of alveoli in SV and MV mice, 21 days after saline tracheal instillation, 14 and 21 days after elastase tracheal instillation. Values are expressed as medians ± interquartile range. n = 3-5 animals/group. ^£ and denote Benjamini-Hochberg corrected p value <0.10 (marginally significant) and <0.05, respectively, for the following Mann-Whitney pairwise comparisons (following Kruskal-Wallis test): Saline SV versus Saline MV, Saline SV versus Ela SV D14, Saline SV versus Ela SV D21, Saline MV versus Ela MV D14, Saline MV versus Ela MV D21, Ela SV versus Ela MV D14, Ela SV versus Ela MV D21, Ela SV D14 versus Ela SV D21, Ela MV D14 versus Ela MV D21. Definition of abbreviations: SV: spontaneous ventilation; MV: mechanical ventilation; Ela: elastase tracheal instillation.

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