Inflammatory Cellular Response to Mechanical Ventilation in Elastase-Induced Experimental Emphysema: Role of Preexisting Alveolar Macrophages Infiltration

Figure 4

Relative values of pulmonary inflammatory cell populations, alveolar macrophages, pulmonary neutrophils, and pulmonary monocytes, analyzed by flow cytometry on total lung cell suspensions of mice subjected to spontaneous (SV) or mechanical (MV) ventilation 14 days (a) and 21 days (b) after instillation of saline (Saline) or elastase (Ela), expressed as percentage of gated cells. Values are expressed as median ± interquartile range. n = 5 animals/group. ^£ and denote Benjamini-Hochberg corrected p value <0.10 (marginally significant) and <0.05, respectively, for the following Mann-Whitney pairwise comparisons (following Kruskal-Wallis test): Saline SV versus Saline MV, Saline SV versus Ela SV, Ela SV versus Ela MV, Saline MV versus Ela MV. Definition of abbreviations: Ela: elastase; SV: spontaneous ventilation; MV: mechanical ventilation.

(a) D14

(b) D21