Small Molecule Inhibitor of Type Three Secretion System Belonging to a Class 2,4-disubstituted-4H-[1,3,4]-thiadiazine-5-ones Improves Survival and Decreases Bacterial Loads in an Airway Pseudomonas aeruginosa Infection in Mice

Figure 6

FT increases the production of inflammatory cytokines in lungs but decreases IL-6 production in blood. A/Sn mice were infected intranasally with 10⁷ CFU/animal of P. aeruginosa exoS⁺ clinical isolate KB6. Mice were treated with 50 mg/kg of FT per os twice daily before infection and once postinfection. IL-6 (a), TNF-alpha (b), and IFN-gamma (c) were tested in lung homogenates at day 1 and 2 PI. IL-6 (d) in blood was tested at day 2 PI. Black bar, treatment with FT; dotted bar, untreated infected mice; grey bar, intact controls, P < 0.05.

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