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BioMed Research International
Volume 2018, Article ID 6147380, 7 pages
https://doi.org/10.1155/2018/6147380
Research Article

Isolation and Molecular Characterization of a Model Antagonistic Pseudomonas aeruginosa Divulging In Vitro Plant Growth Promoting Characteristics

1Department of Bioinformatics and Biotechnology, International Islamic University, Islamabad Capital Territory, Islamabad 44000, Pakistan
2Department of Botany, University of Azad Jammu and Kashmir, Muzaffarabad 13100, Pakistan
3Department of Microbiology, University of Haripur, Haripur, Pakistan
4Department of Botany, University of Gujrat, Hafiz Hayat Campus, Gujrat, Pakistan
5Department of Microbiology, Quaid-i-Azam University, Islamabad 45320, Pakistan
6Department of Forestry and Wild Life Management, University of Haripur, Haripur, Pakistan
7Discipline of Biomedical Science, Sydney Medical School, The University of Sydney, Sydney, NSW, Australia

Correspondence should be addressed to Bushra Uzair; kp.ude.uii@riazu.arhsub

Received 25 August 2017; Accepted 27 November 2017; Published 15 January 2018

Academic Editor: Rida Batool

Copyright © 2018 Bushra Uzair et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The use of microbial technologies in agriculture is currently expanding quite rapidly with the identification of new bacterial strains, which are more effective in promoting plant growth. In the present study 18 strains of Pseudomonas were isolated from soil sample of Balochistan coastline. Among isolated Pseudomonas strains four designated as SP19, SP22, PS24, and SP25 exhibited biocontrol activities against phytopathogenic fungi, that is, Rhizopus microsporus, Fusarium oxysporum, Aspergillus niger, Alternaria alternata, and Penicillium digitatum; PS24 identified as Pseudomonas aeruginosa by 16srRNA gene bank accession number EU081518 was selected on the basis of its antifungal activity to explore its potential as plant growth promotion. PS24 showed multiple plant growth promoting attributes such as phosphate solubilization activity, indole acetic acid (IAA), siderophore, and HCN production. In order to determine the basis for antifungal properties, antibiotics were extracted from King B broth of PS24 and analyzed by TLC. Pyrrolnitrin antibiotic was detected in the culture of strain PS24. PS24 exhibited antifungal activities found to be positive for hydrogen cyanide synthase Hcn BC gene. Sequencing of gene of Hcn BC gene of strain PS24 revealed 99% homology with the Pseudomonas aeruginosa strain PA01. The sequence of PS24 had been submitted in gene bank accession number KR605499. Ps. aeruginosa PS24 with its multifunctional biocontrol possessions can be used to bioprotect the crop plants from phytopathogens.