Effects of Fine Particulate Matter on Pseudomonas aeruginosa Adhesion and Biofilm Formation In Vitro

Figure 2

Three-dimensional depth coding images of a Pseudomonas aeruginosa biofilm were obtained with CLSM after staining with SYTO9/PI. The upper picture in each image panel shows the top view of the biofilm and the lower picture shows the lateral view of the biofilm depth coding. (a) Figure 2(a) shows the top view and lateral view of the biofilm with a thick texture, coarse surface, and red colored protruding biofilm mass after 100 μg/mL FPM treatment. (b, c) The biofilm became thinner and less prominent and the depth of space in the biofilms was shallow after 50 or 25 μg/mL FPM treatment. (d) The thinnest biofilm and shallow nadirs around the projections were on the well without FPM.