Figure 3: Loss of mitochondrial membrane potential (MMP) in HMEECs following FA exposure. (a, b) Histogram of a MitoProbe JC-1 assay shows the decreased red/green fluorescence intensity ratios following cell exposure to FA for 4 h or 24 h. (c) HMEECs exhibited reductions in MMP levels after 4 or 24 h of FA treatment. After 4 h of FA treatment, the reduction in MMP level was statistically significant. Error bars represent SD of the mean. The data represent the means ± SD of three repeated experiments with six samples. compared with control.